Lavender (Lavandula pubescens) is commonly found among the wild flora in the Kingdom of Saudi Arabia (KSA), so calling the "Saudi Wild Lavender" [1]. The lavender has a large part of the aroma and flavor is due to essential oils, some have also biological activity use in traditional medicine. Lavender have been used in medicine, as antimicrobial, antiseptic, and broncho-pulmonary infections therapy [2]. The lavender has antibiofilm activity, which inhibit the formation of biofilm, as antibacterial effects. It has interaction in pathogen cell respiration leads to change in cell respiration causes separation of oxidative phospho-rylation, carbonylation increase bacteriostatic activity so inhibits microbial growth, and has sterile possible. Lipo-philicity and/or hydro-phobicity and hydroxyl groups decisive elements of their antibacterial effect. That reported to lipid membrane lowering activity by interacting with lipo-philic tails of inter-membrane lipid and polar head groups [3]. The lavender has bactericidal effects, antimicrobial activity against twenty five different phytopathogenic bacteria ability to permeabilize and depolarize the cytoplasmic membrane, so inhibit bacterial growth [4]. The lavender methyl ether and Caryophyllene oxide have disintegrated outer membrane of bacterial cells [5]. The lavender has antibacterial action against Salmonella enterica and Staphylococcus aureus [6].

The importance was to use the "Saudi Wild Lavender" oil extracts, which contained powerful substances to eliminate the zoonotic bacteria that transmitted through food to humans, causing food spoilage, loss of food quality, reducing food storage time, and causing humans food poisoning. The goal was to make the "Saudi Wild Lavender" oil extracts and tested against the zoonotic bacteria. The methodology was a laboratory experiment for the materials under study, which included the "Saudi Wild Lavender" oil extracts and the zoonotic bacteria.

Methodology:

Crude oil extract:

The "Saudi Wild Lavender" was obtained from the main area in KSA. The plant parts were clear with distilled water, then were dried in oven at 50°C for loss wetness, and it was electric mixed to reveal powder. The powder 100 g and Ethanol 500 ml were placed in the "Soxhlet Device", for distillation. After extraction procedure, the solvent and extractor were located on water bath for solvent evaporation, and the crude oil extract was resulted [7].

Crude oil dilution:

The crude oil extract was diluted into one-third and two-thirds, was by additional mineral created liquid average has Peptone 5.0g/L, Sodium Chloride 5.0g/L, and Yeast Extract 3.0g/L [8].

Zoonotic bacteria:

The zoonotic bacteria were collected from private laboratory, were identified as the zoonotic bacteria, and were included Staphylococcus sp., Campylobacter jejuni, Escherichia coli, Listeria monocytogenes, and Salmonella sp. The isolates were cultured on Mueller Hinton Agar, and were incubated at 37°C for one night. The pure colonies were transferred to Mueller Hinton Broth and were incubated for four to six hours at 37°C [9].

Laboratory trial:

The sterile capped tubes were prepared, three tubes were used for each extract. Three ml of oil extract and three ml of culture were added to each isolate. The incubation was done for one and two nights at 37°C. The mixture was filtered with the Bacterial Filter Paper, then the dry weight method in grams was performed for the remaining cells after exposure to the oil extract [10].

Data analysis:

The results were obtained, then the arithmetic median was created, and statistics were used, then it was presented in a table to results clarify [11].

Results and discussions:

Table (1): The bacterial dry weight in gram after oil extract interaction